The MNT Antibody (PAC022724) is a highly specific tool for researchers studying the MNT protein, a transcription factor involved in cell cycle regulation and tumorigenesis. This polyclonal antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blotting applications.MNT, a member of the Myc/MAX/MAD transcription factor network, plays a pivotal role in the control of cell proliferation and apoptosis. Dysregulation of MNT has been implicated in various cancers, making it a promising target for therapeutic intervention.
By targeting the MNT protein, researchers can gain insights into its function and potential involvement in disease pathogenesis.This antibody enables precise detection and analysis of MNT expression in different cell types, offering valuable insights into its role in cancer biology and potential therapeutic strategies. With its high specificity and sensitivity, the MNT Antibody is a valuable tool for investigations in oncology and molecular biology research.
Antibody Name:
MNT Antibody (PACO22724)
Antibody SKU:
PACO22724
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human MNT.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from A549 cells, using MNT antibody.
Background:
Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.
Synonyms:
Max-binding protein MNT; Protein ROX; Myc antagonist MNT; MNT; ROX
UniProt Protein Function:
MNT: Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 17p13.3Cellular Component: nucleoplasm; nucleusMolecular Function: protein dimerization activity; transcription coactivator activity; chromatin binding; transcription factor activity; transcription corepressor activityBiological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of cell cycle; multicellular organismal development; cell aging; negative regulation of transcription from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
The Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. This gene encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain. [provided by RefSeq, Jul 2008]
max-binding protein MNT; myc antagonist MNT; MAX binding protein; Max-interacting protein; MNT, MAX dimerization protein; class D basic helix-loop-helix protein 3
UniProt Protein Name:
Max-binding protein MNT
UniProt Synonym Protein Names:
Class D basic helix-loop-helix protein 3; bHLHd3; Myc antagonist MNT; Protein ROX
