The MOB1A/MOB1B Antibody (CAB18246) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications.MOB1A and MOB1B are essential components of the Hippo signaling pathway, which controls organ size and tissue homeostasis. Dysregulation of this pathway has been implicated in various diseases, including cancer.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MOB1A/MOB1B Antibody
SKU:
CAB18246
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein MOB1 is considered to be one of the core components of the mammalian Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. MOB1A and MOB1B, which have 95% sequence identity, play redundant biological roles and are both termed MOB1.
Purification Method
Affinity purification
Gene ID
55233 92597
RRID
AB_2862022
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using MOB1A/MOB1B Rabbit pAb (CAB18246) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates, using MOB1A/MOB2B Rabbit pAb (CAB18246) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
