The Myelin oligodendrocyte glycoprotein Monoclonal Antibody (CAB3992) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting MOG in human samples.MOG is essential for maintaining the structure and function of myelin, the protective sheath surrounding nerve fibers. Dysregulation of MOG has been implicated in various neurological disorders, including multiple sclerosis. By targeting MOG, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify novel therapeutic approaches.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Cell Membrane, Multi-Pass Membrane Protein, Single-Pass Type I Membrane Protein.
Calculated MW:
28kDa
Observed MW:
28kDa
The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
4340
RRID
AB_2863171
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Myelin oligodendrocyte glycoprotein Rabbit mAb (CAB3992) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Myelin oligodendrocyte glycoprotein Rabbit mAb (CAB3992) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Myelin oligodendrocyte glycoprotein Rabbit mAb (CAB3992) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Mouse brain tissue using Myelin oligodendrocyte glycoprotein Rabbit mAb (CAB3992, dilution 1:100) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
