The MOGAT1 Polyclonal Antibody (PACO59277) is a valuable tool for researchers studying MOGAT1, an enzyme involved in lipid metabolism and energy regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the MOGAT1 protein, this antibody facilitates the detection and analysis of MOGAT1 in various cell types, making it an essential resource for studies in metabolic disorders and related research areas.
MOGAT1, also known as monoacylglycerol O-acyltransferase 1, plays a crucial role in lipid biosynthesis and the formation of triglycerides. Its involvement in metabolic pathways makes it a key target for investigation in diseases such as obesity, diabetes, and cardiovascular disorders. Understanding the function of MOGAT1 is essential for developing therapies aimed at managing lipid metabolism and addressing associated health conditions.
IHC image of PACO59277 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO59277 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59277 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine (By similarity).
MOGAT1: Catalyzes the formation of diacylglycerol from 2- monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine. Belongs to the diacylglycerol acyltransferase family.Protein type: Endoplasmic reticulum; Membrane protein, multi-pass; Transferase; Membrane protein, integral; EC 2.3.1.22Chromosomal Location of Human Ortholog: 2q36.1Cellular Component: endoplasmic reticulum membraneMolecular Function: 2-acylglycerol O-acyltransferase activityBiological Process: triacylglycerol biosynthetic process
UniProt Protein Details:
NCBI Summary:
Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerols, the precursor of physiologically important lipids such as triacylglycerol and phospholipids (Yen et al., 2002 [PubMed 12077311]).[supplied by OMIM, Mar 2008]
