The Monkey ACTH (Adrenocorticotropic Hormone) ELISA Kit is specifically designed for the precise measurement of ACTH levels in monkey serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.ACTH is a vital hormone involved in regulating the body's response to stress, controlling cortisol production, and influencing various physiological processes.
Dysregulation of ACTH levels is linked to conditions such as Cushing's disease, adrenal insufficiency, and pituitary adenomas, making it a crucial biomarker for studying these disorders and potentially developing therapeutic interventions. With easy-to-follow protocols and quick assay times, the Monkey ACTH ELISA Kit is an indispensable tool for researchers investigating the role of ACTH in endocrine disorders and exploring potential treatment options.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACTH. During the reaction, ACTH in the sample or standard competes with a fixed amount of ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
99-112
87-98
Average (%)
105
105
93
1:4
Range (%)
89-102
91-102
99-113
Average (%)
96
96
105
1:8
Range (%)
85-98
88-102
95-106
Average (%)
90
94
101
1:16
Range (%)
86-98
88-102
92-105
Average (%)
91
94
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48
106.0
419.0
46.0
102.1
448.7
Standard deviation
2.5
5.7
14.7
3.2
4.4
13.9
C V (%)
5.21
5.38
3.51
6.96
4.31
3.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ACTH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed.
