The Monkey β-CTx (Beta Crosslaps) ELISA Kit is a specialized assay developed to quantitatively measure levels of β-isomerized Crosslinked C-terminal Telopeptides of Type I Collagen (β-CTx) in monkey biological samples. β-CTx is recognized as a biochemical marker of bone resorption, reflecting the degradation of type I collagen in bone tissues, making it an essential biomarker for studying bone metabolism and turnover in non-human primates.
This ELISA kit enables researchers to accurately assess β-CTx levels in monkey samples, providing valuable information on bone resorption rates, osteoporosis, and skeletal health in primate models. By quantifying β-CTx, researchers can gain insights into bone remodeling processes, bone mineral density changes, and the efficacy of therapeutic interventions targeting bone-related disorders in monkeys. With exceptional sensitivity and specificity, the Monkey β-CTx ELISA Kit offers reliable and reproducible results, empowering researchers to investigate bone turnover dynamics and assess the impact of various factors on bone health. Manufactured under stringent quality control standards, this kit ensures robust performance and user-friendly protocols, making it a valuable tool for studying skeletal metabolism and diseases in monkey research models.
Product Name:
Monkey beta-CTx (Beta Crosslaps) ELISA Kit
SKU:
AEES00689
Target:
Monkey β-CTx (Beta Crosslaps)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-CTx. During the reaction, β-CTx in the sample or standard competes with a fixed amount of β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
94-110
84-96
Average (%)
97
101
90
1:4
Range (%)
92-108
94-112
82-95
Average (%)
98
102
88
1:8
Range (%)
87-99
94-109
82-94
Average (%)
94
102
89
1:16
Range (%)
89-103
93-105
86-101
Average (%)
96
98
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
441.29
936.13
2891.85
477.86
968.26
2650.94
Standard deviation
23.12
37.73
155.58
31.83
54.61
139.17
C V (%)
5.24
4.03
5.38
6.66
5.64
5.25
Sample type &Sample volume:
; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of β-CTx concentrations in .
Specificity:
This kit recognizes β-CTx in samples. No significant cross-reactivity or interference between β-CTx and analogues was observed.
