The Monkey FcGamma (Fc Fragment of IgG) ELISA Kit is a cutting-edge tool designed for the quantitative detection of IgG Fc fragment in monkey serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.The IgG Fc fragment plays a vital role in immune responses and antibody-mediated functions, making it a crucial biomarker for studying immune system disorders, infections, and autoimmune diseases in monkeys.
By accurately measuring IgG Fc fragment levels, researchers can gain valuable insights into immune function and potentially develop new therapeutic strategies for various health conditions affecting monkeys.
Product Name:
Monkey Fcgamma (Fc Fragment of IgG) ELISA Kit
SKU:
MKES00008
Target:
Monkey Fcgamma (Fc Fragment of IgG)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey Fcγ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey Fcγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey Fcγ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey Fcγ. You can calculate the concentration of Monkey Fcγ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
88-100
94-109
Average (%)
92
94
100
1:4
Range (%)
84-94
89-101
92-104
Average (%)
89
96
98
1:8
Range (%)
87-100
84-98
89-100
Average (%)
92
90
95
1:16
Range (%)
83-97
84-98
93-107
Average (%)
90
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
84-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
1.77
7.36
0.99
1.91
7.99
Standard deviation
0.06
0.08
0.4
0.07
0.09
0.42
C V (%)
5.88
4.52
5.43
7.07
4.71
5.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey Fcγ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey Fcγ in samples. No significant cross-reactivity or interference between Monkey Fcγ and analogues was observed.
