The Monkey Gremlin 1 (GREM1) ELISA Kit is a specialized assay developed for the precise and quantitative measurement of Gremlin 1 levels in monkey samples. Gremlin 1 is a critical regulatory protein that acts as a bone morphogenetic protein antagonist, modulating cell growth, development, and differentiation processes in primates. This ELISA kit offers researchers a reliable method to quantitatively assess Gremlin 1 expression in monkey biological samples, providing valuable insights into its role in embryonic development, tissue homeostasis, and disease pathogenesis in non-human primates. By accurately measuring GREM1 levels, researchers can investigate the impact of this crucial protein on cellular signaling, organogenesis, and various diseases in primate models.
With exceptional sensitivity and specificity, the Monkey GREM1 ELISA Kit ensures accurate and reproducible results, enabling comprehensive studies on the functional significance of Gremlin 1 in developmental biology, regenerative medicine, and disease mechanisms in monkey research. Manufactured under stringent quality control measures, this kit delivers reliable performance and user-friendly protocols, making it an excellent tool for studying GREM1-related pathways in primate models.
Product Name:
Monkey GREM1 (Gremlin 1) ELISA Kit
SKU:
AEES00687
Target:
Monkey GREM1 (Gremlin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey GREM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey GREM1. You can calculate the concentration of Monkey GREM1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
97-111
89-105
Average (%)
92
105
96
1:4
Range (%)
86-97
94-110
85-98
Average (%)
91
101
91
1:8
Range (%)
88-98
98-115
90-105
Average (%)
93
106
96
1:16
Range (%)
82-94
95-111
90-104
Average (%)
89
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.32
3.41
0.48
1.27
3.57
Standard deviation
0.03
0.06
0.17
0.03
0.07
0.19
C V (%)
6.12
4.55
4.99
6.25
5.51
5.32
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey GREM1 concentrations in .
Specificity:
This kit recognizes Monkey GREM1 in samples. No significant cross-reactivity or interference between Monkey GREM1 and analogues was observed.
