The Monkey NEFL (Neurofilament, Light Polypeptide) ELISA Kit is a specialized assay designed to quantitatively measure NEFL levels in monkey biological samples. NEFL is an essential protein found predominantly in neurons and is a critical component of the neuronal cytoskeleton. Changes in NEFL levels can provide valuable insights into neuronal function and integrity, making it a key marker for neurological research.
This ELISA kit enables researchers to accurately assess NEFL levels in monkey samples, facilitating investigations into neurodegenerative diseases, neuronal injuries, and neurological disorders. NEFL is particularly relevant in the context of neurology and neuroscience, as alterations in its expression can indicate neuronal damage or dysfunction. With exceptional sensitivity and specificity, the Monkey NEFL ELISA Kit ensures precise and reproducible results, allowing researchers to gain important information about neuronal health and neurological conditions in primate models. Manufactured under strict quality control measures, this kit offers reliable performance and user-friendly protocols, making it an ideal choice for studies focusing on neurobiology and neurodegeneration in monkeys.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey NEFL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey NEFL. You can calculate the concentration of Monkey NEFL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
90-100
86-98
Average (%)
93
95
93
1:4
Range (%)
83-98
89-101
86-100
Average (%)
90
96
93
1:8
Range (%)
85-98
87-100
85-99
Average (%)
92
94
90
1:16
Range (%)
89-103
87-97
85-98
Average (%)
95
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.98
172.69
757.85
92.68
162.57
689.19
Standard deviation
5.1
7.15
30.84
6.27
8.27
26.53
C V (%)
5.49
4.14
4.07
6.77
5.09
3.85
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey NEFL concentrations in .
Specificity:
This kit recognizes Monkey NEFL in samples. No significant cross-reactivity or interference between Monkey NEFL and analogues was observed.
