The Monkey OPN (Osteopontin) ELISA Kit is specifically designed for the precise measurement of Osteopontin levels in monkey serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.Osteopontin is a key protein that plays a crucial role in numerous biological processes, including cell adhesion, migration, and signaling. It is implicated in various pathological conditions such as inflammation, cancer, and atherosclerosis, making it a valuable biomarker for studying and understanding these diseases.
With the Monkey OPN (Osteopontin) ELISA Kit, researchers can uncover new insights into the role of Osteopontin in disease progression and potentially identify new therapeutic targets for treatment. Trust in the reliability and precision of this ELISA kit for your research needs.
Product Name:
Monkey OPN (Osteopontin) ELISA Kit
SKU:
MKES00033
Target:
Monkey OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey OPN. You can calculate the concentration of Monkey OPN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
94-106
92-109
Average (%)
93
100
99
1:4
Range (%)
89-102
95-110
94-105
Average (%)
95
102
100
1:8
Range (%)
85-98
95-109
91-104
Average (%)
91
100
98
1:16
Range (%)
85-99
93-106
88-102
Average (%)
90
98
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-95
90
EDTA plasma (n=5)
90-107
97
Cell culture media (n=5)
94-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
2.13
9.07
1.12
2.0
9.71
Standard deviation
0.06
0.09
0.37
0.06
0.09
0.3
C V (%)
5.56
4.23
4.08
5.36
4.5
3.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey OPN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey OPN in samples. No significant cross-reactivity or interference between Monkey OPN and analogues was observed.
