The Monkey PCSK9 ELISA Kit is a specialized assay designed for the accurate and sensitive quantitative measurement of Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) levels in monkey biological samples. PCSK9 is a crucial regulator of low-density lipoprotein receptor metabolism, impacting cholesterol homeostasis and cardiovascular health in non-human primates. This ELISA kit offers researchers a precise method to quantify PCSK9 expression in monkey samples, providing valuable insights into the role of PCSK9 in lipid metabolism, atherosclerosis, and cardiovascular disease models. By quantifying PCSK9 levels, researchers can investigate its involvement in the regulation of cholesterol levels and the development of cardiovascular conditions in monkey models.
With exceptional sensitivity and specificity, the Monkey PCSK9 ELISA Kit enables reliable and reproducible results, facilitating insightful studies on PCSK9-mediated pathways in primate research. Manufactured under stringent quality control standards, this kit ensures robust performance and user-friendly protocols, making it an ideal tool for exploring the functions of PCSK9 in monkey cardiovascular health and metabolic disorders.
Product Name:
Monkey PCSK9 ELISA Kit
SKU:
AEES00688
Target:
Monkey PCSK9
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey PCSK9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey PCSK9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey PCSK9. You can calculate the concentration of Monkey PCSK9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
93-105
85-98
Average (%)
103
99
91
1:4
Range (%)
93-103
95-112
83-94
Average (%)
98
102
89
1:8
Range (%)
97-112
94-110
89-103
Average (%)
104
101
94
1:16
Range (%)
95-107
90-105
88-101
Average (%)
101
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.44
7.95
21.89
2.64
7.56
23.56
Standard deviation
0.16
0.37
1.07
0.14
0.36
1.1
C V (%)
6.56
4.65
4.89
5.3
4.76
4.67
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey PCSK9 concentrations in .
Specificity:
This kit recognizes Monkey PCSK9 in samples. No significant cross-reactivity or interference between Monkey PCSK9 and analogues was observed.
