The Monkey SAA (Serum Amyloid A) ELISA Kit is a specialized assay designed for the accurate and quantitative measurement of SAA levels in monkey biological samples.
Serum Amyloid A is a major acute-phase protein involved in various inflammatory processes and immune responses, making it an important biomarker for assessing inflammation and infection. This ELISA kit enables researchers to detect SAA concentrations in monkey samples, providing valuable insights into the dynamics of the acute-phase response, inflammatory conditions, and immune system activation in non-human primates. By quantifying SAA levels, researchers can study the pathophysiology of inflammatory diseases, monitor disease progression, and evaluate treatment efficacy in primate models. With exceptional sensitivity and specificity, the Monkey SAA ELISA Kit delivers reliable and reproducible results, allowing researchers to investigate SAA's role in inflammation, infection, and immune modulation in monkeys. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly protocols, making it a valuable tool for research in primate immunology and inflammatory disorders.
Product Name:
Monkey SAA (Serum Amyloid A) ELISA Kit
SKU:
AEES00719
Target:
Monkey SAA (Serum Amyloid A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey SAA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey SAA. You can calculate the concentration of Monkey SAA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
91-107
96-110
Average (%)
95
97
101
1:4
Range (%)
85-99
88-102
93-111
Average (%)
91
96
101
1:8
Range (%)
90-103
90-105
95-111
Average (%)
97
98
101
1:16
Range (%)
89-101
93-107
90-102
Average (%)
94
98
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.94
305.41
733.79
99.2
291.64
710.05
Standard deviation
5.4
15.42
25.24
5.45
12.89
30.89
C V (%)
5.2
5.05
3.44
5.49
4.42
4.35
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey SAA concentrations in .
Specificity:
This kit recognizes Monkey SAA in samples. No significant cross-reactivity or interference between Monkey SAA and analogues was observed.
