The Monomethyl Histone H4 (K5) Polyclonal Antibody (CAB17859) is a valuable tool for researchers studying epigenetics and chromatin remodeling processes. This antibody, raised in rabbits, specifically targets the monomethylated form of histone H4 at lysine 5, a crucial modification involved in gene regulation and chromatin structure.Histone modifications, such as methylation at lysine residues, play a key role in regulating gene expression by altering the accessibility of DNA to transcription factors and other regulatory proteins. The Monomethyl Histone H4 (K5) Polyclonal Antibody enables researchers to detect and analyze this specific histone modification in various cell types and experimental conditions.
By studying the role of monomethylated histone H4 at lysine 5, researchers can gain insights into how epigenetic changes impact cellular processes, including differentiation, development, and disease. This antibody is validated for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence, making it a versatile tool for investigating the role of histone modifications in biological pathways.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H4-K5 Rabbit pAb (CAB17859) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
