The Mouse 5-LO (Arachidonate 5-Lipoxygenase) CLIA Kit is a reliable assay designed for the accurate detection of 5-LO levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.5-LO is an important enzyme involved in the metabolism of arachidonic acid, playing a crucial role in inflammatory responses and lipid mediator production.
Dysregulation of 5-LO has been implicated in various inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutics.Overall, the Mouse 5-LO CLIA Kit is a valuable tool for researchers studying the role of 5-LO in inflammation, oxidative stress, and related pathways in mouse models.
Product Name:
Mouse 5-LO (Arachidonate 5-Lipoxygenase) CLIA Kit
SKU:
MOES00605
Target:
Mouse 5-LO (Arachidonate 5-Lipoxygenase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse 5-LO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse 5-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse 5-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse 5-LO. You can calculate the concentration of Mouse 5-LO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
88-101
98-115
Average (%)
105
95
105
1:4
Range (%)
95-111
101-118
96-111
Average (%)
102
108
102
1:8
Range (%)
93-107
96-108
84-99
Average (%)
100
103
91
1:16
Range (%)
91-104
97-114
102-118
Average (%)
96
105
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
85-97
92
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.25
310.32
873.25
95.24
338.73
822.02
Standard deviation
10.37
34.2
86.98
10.95
23.98
71.35
C V (%)
9.85
11.02
9.96
11.5
7.08
8.68
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse 5-LO concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Mouse 5-LO in samples. No significant cross-reactivity or interference between Mouse 5-LO and analogues was observed.
