The Mouse ABCA1 (ATP Binding Cassette Transporter A1) CLIA Kit is a reliable and accurate tool for the detection of ABCA1 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ABCA1 is a critical transporter protein involved in cholesterol efflux and plays a key role in lipid metabolism and inflammation. Dysregulation of ABCA1 is associated with various diseases including atherosclerosis, Alzheimer's disease, and diabetes.
Therefore, monitoring ABCA1 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Mouse ABCA1 CLIA Kit is a valuable tool for researchers studying lipid metabolism, inflammation, and related diseases in mouse models. Its high performance and reliability make it an essential component of any laboratory focused on understanding the role of ABCA1 in health and disease.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ABCA1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ABCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ABCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ABCA1. You can calculate the concentration of Mouse ABCA1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
102-118
85-96
Average (%)
107
108
91
1:4
Range (%)
100-115
102-120
95-108
Average (%)
108
109
101
1:8
Range (%)
95-106
91-105
102-117
Average (%)
101
96
108
1:16
Range (%)
90-105
87-97
94-108
Average (%)
96
92
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
100-116
108
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.93
228.12
962.78
98.64
214.67
976.57
Standard deviation
8.93
22.49
75.29
10.52
16.31
61.72
C V (%)
9.31
9.86
7.82
10.67
7.6
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ABCA1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ABCA1 in samples. No significant cross-reactivity or interference between Mouse ABCA1 and analogues was observed.
