The Mouse ACTH (Adrenocorticotropic Hormone) ELISA Kit is a reliable and accurate tool for detecting levels of ACTH in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides dependable and reproducible results for a variety of research applications.ACTH is a vital hormone that stimulates the production and release of cortisol from the adrenal glands. Dysregulation of ACTH levels can lead to various health conditions such as Cushing's syndrome and Addison's disease. Monitoring ACTH levels is essential for understanding these disorders and developing effective treatments.Whether studying hormone regulation, stress response, or adrenal function, the Mouse ACTH ELISA Kit is an invaluable research tool for investigating the intricate mechanisms of hormone signaling and potential therapeutic interventions.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
