The Rat Adrenocorticotropic Hormone (ACTH) ELISA Kit is meticulously crafted for the precise and quantitative measurement of ACTH levels in various rat biological samples. ACTH, a pivotal hormone produced by the pituitary gland, plays a critical role in regulating cortisol secretion from the adrenal glands. This hormone is essential for the body's stress response, metabolism, and overall homeostasis. This state-of-the-art ELISA kit empowers researchers to explore the dynamics of ACTH levels, providing valuable insights into its role in stress responses, adrenal function, and potential implications in a range of physiological and pathological conditions. With exceptional sensitivity and specificity, this assay ensures accurate and reproducible results, offering a reliable platform for investigating ACTH biology. Manufactured under stringent quality control standards, this kit delivers robust performance, making it a reliable and user-friendly tool for scientific research endeavors.
Product Name:
Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit
SKU:
AEES00308
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
