Mouse AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit
The Mouse AGER (Advanced Glycosylation End Product-specific Receptor) CLIA Kit is a highly accurate and sensitive assay designed for the detection of AGER levels in mouse serum, plasma, and cell culture supernatants. This kit is specifically developed to provide reliable and reproducible results, making it ideal for a wide range of research applications.AGER is a key receptor involved in the recognition and clearance of advanced glycation end products, which are implicated in various diseases such as diabetes, cardiovascular diseases, and chronic inflammation.
By measuring AGER levels, researchers can gain valuable insights into the role of this receptor in disease pathogenesis and progression, facilitating the development of potential therapeutic interventions.Overall, the Mouse AGER CLIA Kit offers researchers a valuable tool for studying the role of AGER in disease biology and for identifying novel therapeutic targets for various conditions.
Product Name:
Mouse AGER (Advanced Glycosylation End Product Specific Receptor) CLIA Kit
SKU:
MOES00096
Target:
Mouse AGER (Advanced Glycosylation End Product Specific Receptor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AGER. You can calculate the concentration of Mouse AGER in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
93-107
96-111
Average (%)
92
100
102
1:4
Range (%)
94-105
97-112
101-117
Average (%)
100
105
108
1:8
Range (%)
87-101
90-103
92-103
Average (%)
92
95
98
1:16
Range (%)
94-109
90-102
97-110
Average (%)
100
95
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.96
198.09
856.22
89.37
211.4
804.81
Standard deviation
9.21
17.21
84.42
9.87
20.21
75.17
C V (%)
10.13
8.69
9.86
11.04
9.56
9.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AGER concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AGER in samples. No significant cross-reactivity or interference between Mouse AGER and analogues was observed.
