The Mouse AHR (Aryl Hydrocarbon Receptor) CLIA Kit is a high-quality assay designed for the accurate quantification of AHR levels in mouse serum, plasma, and cell lysates. This kit provides exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.The aryl hydrocarbon receptor (AHR) is a critical cellular protein involved in the regulation of gene expression and plays a crucial role in various biological processes, including immune response, metabolism, and development.
Dysregulation of AHR signaling has been implicated in a range of diseases, such as cancer, autoimmune disorders, and metabolic syndromes, making it a valuable target for therapeutic interventions.With its reliability and efficiency, the Mouse AHR CLIA Kit is an essential tool for researchers to study AHR biology, explore its role in disease pathogenesis, and develop potential therapeutic strategies.
Product Name:
Mouse AhR (Aryl Hydrocarbon Receptor) CLIA Kit
SKU:
MOES00100
Target:
Mouse AhR (Aryl Hydrocarbon Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AhR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AhR. You can calculate the concentration of Mouse AhR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
84-96
94-109
Average (%)
107
90
101
1:4
Range (%)
100-115
95-109
102-119
Average (%)
106
102
110
1:8
Range (%)
86-98
99-115
87-98
Average (%)
91
107
92
1:16
Range (%)
96-111
101-113
86-102
Average (%)
103
107
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
99-112
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
239.73
772.25
2273.22
236.89
830.97
2346.55
Standard deviation
28.26
65.87
218.23
24.78
59.33
269.62
C V (%)
11.79
8.53
9.6
10.46
7.14
11.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AhR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AhR in samples. No significant cross-reactivity or interference between Mouse AhR and analogues was observed.
