Mouse Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) ELISA Kit
The Mouse ALDH7A1 ELISA Kit is specifically designed for the precise measurement of mouse aldehyde dehydrogenase 7 family member A1 (ALDH7A1) levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.ALDH7A1 is an essential enzyme involved in the metabolism of aldehydes and amino acids, playing a critical role in various metabolic pathways. Dysregulation of ALDH7A1 has been linked to conditions such as pyridoxine-dependent epilepsy and other metabolic disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With the Mouse ALDH7A1 ELISA Kit, researchers can confidently analyze ALDH7A1 levels in mouse samples, advancing our understanding of its biological functions and implications in disease pathology. Trust in the reliability and performance of this kit for your research needs.
Product Name:
Mouse Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
