Mouse Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) ELISA Kit
The Mouse ALDH9A1 ELISA Kit is specifically designed for the precise quantification of mouse aldehyde dehydrogenase 9 family member A1 (ALDH9A1) levels in various biological samples, including serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a wide range of research applications.ALDH9A1 is an essential enzyme involved in the detoxification of aldehydes and plays a critical role in cellular metabolism.
Dysregulation of ALDH9A1 has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. Therefore, the quantification of ALDH9A1 levels can provide valuable insights into disease progression and potential therapeutic strategies.Overall, the Mouse ALDH9A1 ELISA Kit is a valuable tool for researchers studying the role of ALDH9A1 in health and disease, offering a reliable and efficient method for analyzing ALDH9A1 levels in experimental samples.
Product Name:
Mouse Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
