The Mouse Alpha1-mg (Alpha1-Microglobulin) CLIA Kit is specifically designed for the accurate measurement of alpha1-microglobulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.Alpha1-microglobulin is a critical protein involved in renal function and oxidative stress response. It serves as a valuable biomarker for monitoring kidney health and identifying potential renal complications.
Additionally, it has been implicated in various diseases such as diabetes, sepsis, and kidney injury, highlighting its importance in biomedical research and therapeutic development. With its advanced technology and ease of use, the Mouse Alpha1-mg CLIA Kit is an indispensable tool for researchers studying renal diseases, oxidative stress, and related pathologies in mouse models.
Product Name:
Mouse alpha1-MG (alpha1-Microglobulin) CLIA Kit
SKU:
MOES00598
Target:
Mouse alpha1-MG (alpha1-Microglobulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse α1-MG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse α1-MG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse α1-MG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse α1-MG. You can calculate the concentration of Mouse α1-MG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
101-117
90-102
Average (%)
100
108
95
1:4
Range (%)
95-112
93-104
89-104
Average (%)
103
98
95
1:8
Range (%)
104-118
97-108
89-103
Average (%)
110
103
95
1:16
Range (%)
99-113
94-108
94-106
Average (%)
106
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
104
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
94-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.14
7.9
1.11
2.12
8.1
Standard deviation
0.13
0.2
0.65
0.13
0.17
0.62
C V (%)
11.93
9.35
8.23
11.71
8.02
7.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse α1-MG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse α1-MG in samples. No significant cross-reactivity or interference between Mouse α1-MG and analogues was observed.
