The Mouse AMH (Anti-Mullerian Hormone) ELISA Kit is carefully developed to facilitate the precise quantitative measurement of Anti-Mullerian Hormone levels in various mouse biological samples. Anti-Mullerian Hormone (AMH) is a key regulator of reproductive processes and is primarily known for its role in the development of male and female reproductive organs. AMH levels are indicative of the ovarian reserve and can be utilized as a biomarker for assessing fertility, reproductive maturity, and certain reproductive disorders.
With superior sensitivity and specificity, Assay Genie's Mouse AMH ELISA Kit ensures accurate and reproducible results that are essential for in-depth investigations into reproductive biology, fertility assessment, and related conditions. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for research studies focused on the intricate mechanisms of reproductive health.
Product Name:
Mouse AMH (Anti-Mullerian Hormone) ELISA Kit
SKU:
AEES00210
Target:
Mouse AMH (Anti-Mullerian Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AMH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AMH. You can calculate the concentration of Mouse AMH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-99
101-109
100-111
Average (%)
93
104
104
1:4
Range (%)
96-109
100-110
96-109
Average (%)
102
105
104
1:8
Range (%)
96-110
93-102
93-103
Average (%)
103
97
100
1:16
Range (%)
95-106
83-94
90-101
Average (%)
101
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.17
6.14
21.57
2.24
5.09
24.21
Standard deviation
0.12
0.28
1.11
0.13
0.25
1.07
C V (%)
5.53
4.56
5.15
5.8
4.91
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse AMH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AMH in samples. No significant cross-reactivity or interference between Mouse AMH and analogues was observed.
