The Mouse ANG (Angiogenin) CLIA Kit is a powerful tool for accurate and reliable detection of angiogenin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Angiogenin is a key protein involved in angiogenesis, playing a crucial role in blood vessel formation and cell proliferation. Its significance in conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders makes it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With the Mouse ANG CLIA Kit, researchers can confidently measure angiogenin levels in mouse samples, providing valuable insights into the mechanisms underlying disease progression and potential treatment strategies. Trust this kit for your research needs and unlock new possibilities in the field of angiogenin research.
Product Name:
Mouse ANG (Angiogenin) CLIA Kit
SKU:
MOES00061
Target:
Mouse ANG (Angiogenin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANG. You can calculate the concentration of Mouse ANG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-119
86-97
94-109
Average (%)
109
91
101
1:4
Range (%)
93-105
97-114
96-112
Average (%)
99
104
103
1:8
Range (%)
95-107
91-103
103-119
Average (%)
101
98
110
1:16
Range (%)
103-116
100-114
90-105
Average (%)
109
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.82
155.34
432.63
54.74
149.29
449.57
Standard deviation
5.64
13.75
32.97
6.43
11.14
47.56
C V (%)
10.48
8.85
7.62
11.75
7.46
10.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANG in samples. No significant cross-reactivity or interference between Mouse ANG and analogues was observed.
