The Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit is a powerful tool for the sensitive and accurate detection of Ang1-7 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Angiotensin 1-7 is a key component of the renin-angiotensin system, playing a crucial role in cardiovascular regulation, blood pressure control, and inflammation. Dysregulation of Ang1-7 levels is associated with cardiovascular diseases, hypertension, and kidney disorders, highlighting its importance as a potential therapeutic target.
By using the Mouse Ang1-7 ELISA Kit, researchers can gain valuable insights into the role of Ang1-7 in various physiological and pathological processes, aiding in the development of novel treatments and interventions. With its user-friendly and efficient design, this kit is a valuable asset for studies focused on understanding the complex mechanisms of Ang1-7 signaling.
Product Name:
Mouse Ang1-7 (Angiotensin 1-7) ELISA Kit
SKU:
MOES01816
Target:
Mouse Ang1-7 (Angiotensin 1-7) ELISA Kit
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang1-7. During the reaction, Ang1-7 in the sample or standard competes with a fixed amount of Ang1-7 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang1-7. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang1-7 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
92-106
87-103
Average (%)
105
98
94
1:4
Range (%)
90-102
94-105
97-110
Average (%)
96
99
103
1:8
Range (%)
87-97
87-103
95-112
Average (%)
92
94
102
1:16
Range (%)
85-101
90-105
95-108
Average (%)
92
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
92-108
98
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.9
181.4
856.3
103.2
184.2
774.7
Standard deviation
6.7
9.4
31.7
5.4
7.4
30.2
C V (%)
6.91
5.18
3.7
5.23
4.02
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Ang1-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Ang1-7 in samples. No significant cross-reactivity or interference between Ang1-7 and analogues was observed.
