The Mouse ANG1 (Angiopoietin-1) CLIA Kit is specifically designed for the accurate measurement of Angiopoietin-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it an invaluable tool for various research applications.Angiopoietin-1 is a key factor in angiogenesis, promoting the formation of blood vessels and impacting cell growth.
Its role in various diseases such as cancer, cardiovascular disorders, and neurological conditions underscores its importance as a biomarker for research and potential therapeutic development.With the Mouse ANG1 CLIA Kit, researchers can confidently study the role of Angiopoietin-1 in disease progression and treatment, leading to a better understanding of these complex conditions and the development of targeted therapies.
Product Name:
Mouse ANG1 (Angiopoietin 1) CLIA Kit
SKU:
MOES00074
Target:
Mouse ANG1 (Angiopoietin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANG1. You can calculate the concentration of Mouse ANG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
90-104
91-106
Average (%)
104
97
96
1:4
Range (%)
100-118
94-108
92-103
Average (%)
107
101
98
1:8
Range (%)
86-99
83-98
87-101
Average (%)
91
90
94
1:16
Range (%)
100-116
91-104
104-116
Average (%)
107
97
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
94-106
99
Cell culture media (n=5)
101-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.32
311.65
904.76
115.53
334.93
963.21
Standard deviation
8.78
27.02
86.04
11.61
35.64
94.78
C V (%)
8.11
8.67
9.51
10.05
10.64
9.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANG1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANG1 in samples. No significant cross-reactivity or interference between Mouse ANG1 and analogues was observed.
