The Mouse ANG3 (Angiopoietin-3) CLIA Kit is specifically designed for the precise measurement of Angiopoietin-3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, producing highly reliable and consistent results, thus making it an excellent choice for various research applications.Angiopoietin-3 is a key protein involved in regulating blood vessel formation and affecting cell growth. Its crucial role in conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders makes it a valuable biomarker for investigating these diseases and developing potential therapeutic interventions.
This kit is a valuable tool for researchers studying angiogenesis and its role in various diseases, providing accurate and reliable data for further exploration and understanding of Angiopoietin-3's physiological and pathological functions.
Product Name:
Mouse ANG3 (Angiopoietin 3) CLIA Kit
SKU:
MOES00003
Target:
Mouse ANG3 (Angiopoietin 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANG3. You can calculate the concentration of Mouse ANG3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
94-110
89-103
Average (%)
107
100
96
1:4
Range (%)
90-103
87-101
103-114
Average (%)
97
93
108
1:8
Range (%)
103-116
92-107
90-103
Average (%)
110
99
97
1:16
Range (%)
95-113
103-118
99-111
Average (%)
103
109
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
94-108
100
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.2
176.75
846.16
105.07
167.6
905.29
Standard deviation
12.79
19.39
54.66
11.48
14.97
63.55
C V (%)
11.61
10.97
6.46
10.93
8.93
7.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANG3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANG3 in samples. No significant cross-reactivity or interference between Mouse ANG3 and analogues was observed.
