Mouse Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit
The Mouse Angiopoietin-Like Protein 2 (ANGPTL2) ELISA Kit is specifically designed for the precise measurement of ANGPTL2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.ANGPTL2 is a key protein involved in regulating angiogenesis and inflammation, playing a critical role in various diseases such as obesity, diabetes, and cardiovascular disorders.
By detecting ANGPTL2 levels, researchers can better understand the role of this protein in disease progression and potentially develop targeted therapies for these conditions.Overall, the Mouse ANGPTL2 ELISA Kit is a valuable tool for studying the role of ANGPTL2 in disease pathogenesis and identifying potential therapeutic targets for various health conditions.
Product Name:
Mouse Angiopoietin Like Protein 2 (ANGPTL2) ELISA Kit
Product Code:
MODL00072
Size:
96 Assays
Target:
ANGPTL2
Synonyms:
HARP, ARP2, Angiopoietin-Related Protein 2
Detection Method:
Sandwich
Range:
6.25-400pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
