Description
Mouse anti GST-Tag mAb (CABE001)
The Mouse anti GST-Tag mAb (CABE001) is a high-quality antibody developed for reliable detection and analysis of target proteins. Glutathione S-transferases (GSTs) are a family of phase II metabolic isozymes that catalyze the conjugation of glutathione to xenobiotic substrates for detoxification, comprising cytosolic, mitochondrial, and microsomal subfamilies. The GST-tag (~26 kDa) is widely used in recombinant protein production as a solubility-enhancing partner and as an affinity handle for purification via glutathione-Sepharose chromatography.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Species independent samples.
| Product Name: | Mouse anti GST-Tag mAb |
| SKU: | CABE001 |
| Size: | 50μL, 100μL, 200μL |
| Reactivity: | Species independent |
| Clone Number: | AMC0501 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||
| Tested Applications: | WB IP ELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | GST, GST tag, GST-tag |
| Positive Sample: | GST-Tag |
| Calculated MW: | 26kDa |
| Observed MW: | 27kDa |
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
| Purification Method | Affinity purification |
| RRID | AB_2770403 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 1.5% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of over-expressed GST protein using Mouse anti GST-Tag mAb (CABE001) at different dilution. Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 1 μg extracts of GST-Tag cells using 3 μg Mouse anti GST-Tag mAb antibody (CABE001). Western blot was performed from the immunoprecipitate using Mouse anti GST-Tag mAb antibody (CABE001) at a dilution of 1:1000. |