The Mouse anti MBP-Tag Monclonal Antibody (CABE016) is a high-quality antibody developed for reliable detection and analysis of target proteins. MBP (Maltose-Binding Protein, ~42.5 kDa) is an E. coli periplasmic protein responsible for maltodextrin uptake and catabolism that is widely used as a recombinant fusion tag to enhance the soluble expression of aggregation-prone proteins in bacterial systems. It enables single-step affinity purification via amylose resin under mild elution conditions that preserve protein activity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Mouse anti MBP-Tag Monclonal Antibody
SKU:
CABE016
Size:
100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0505
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000-1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MBP, MBP tag, MBP-tag
Positive Sample:
Recombinant MBP protein, E. coli
Calculated MW:
42.5kDa
Observed MW:
42kDa/40kDa
Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons.
Purification Method
Affinity purification
RRID
AB_2770406
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from E. coli cells using Mouse anti MBP-Tag mAb (CABE016) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25 ng/10 ng/1 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Western blot analysis of recombinant MBP protein using Mouse anti MBP-Tag mAb (CABE016) at dilution of 1:5000.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
