Description
Mouse anti mCherry-Tag Monclonal Antibody (CABE002)
The Mouse anti mCherry-Tag Monclonal Antibody (CABE002) is a high-quality antibody developed for reliable detection and analysis of target proteins. mCherry is a monomeric red fluorescent protein engineered from the Discosoma coral DsRed that emits stable red fluorescence without dimerization artifacts, making it well suited for fusion tagging of proteins of interest. It is widely used in dual-color live-cell imaging due to its clear spectral separation from GFP and other commonly used fluorescent reporters.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
| Product Name: | Mouse anti mCherry-Tag Monclonal Antibody |
| SKU: | CABE002 |
| Size: | 50μL, 100μL, 200μL |
| Reactivity: | Species independent |
| Clone Number: | AMC0502 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | mCherry, mCherry tag, mCherry-tag |
| Positive Sample: | 293T-CT55-mcherry |
| Observed MW: | 55kDa |
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
| Purification Method | Affinity purification |
| RRID | AB_2770407 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 1% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with CT55 Protein, using Mouse anti mCherry-Tag mAb (CABE002) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s. |