Description
Mouse anti Trx-Tag Monclonal Antibody (CABE023)
The Mouse anti Trx-Tag Monclonal Antibody (CABE023) is a high-quality antibody developed for reliable detection and analysis of target proteins. Thioredoxins are a conserved family of small redox proteins present in all organisms that regulate cellular redox state and play central roles in redox signaling pathways. The Trx-tag (E. coli thioredoxin) is used as a recombinant fusion partner to enhance solubility and assist in the correct folding of disulfide bond-containing proteins during bacterial expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
| Product Name: | Mouse anti Trx-Tag Monclonal Antibody |
| SKU: | CABE023 |
| Size: | 100μL, 200μL |
| Reactivity: | Species independent |
| Clone Number: | AMC0511 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | TRX, TRX tag, TRX-tag |
| Observed MW: | RefertoFigures |
Thioredoxin is a class of small redox proteins known to be present in all organisms. It plays a role in many important biological processes, including redox signaling. In humans, thioredoxins are encoded by TXN and TXN2 genes. Loss-of-function mutation of either of the two human thioredoxin genes is lethal at the four-cell stage of the developing embryo. Although not entirely understood, thioredoxin plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). In plants, thioredoxins regulate a spectrum of critical functions, ranging from photosynthesis to growth, flowering and the development and germination of seeds. It has also recently been found to play a role in cell-to-cell communication.
| Purification Method | Affinity purification |
| RRID | AB_2770412 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of over-expressed Trx fusion protein using Mouse anti Trx-Tag mAb (CABE023) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. |