The Mouse ANXA2 (Annexin A2) CLIA Kit is specifically designed for the precise measurement of Annexin A2 levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for various research applications.Annexin A2 is a key protein involved in a variety of cellular processes, including membrane trafficking, cell adhesion, and signal transduction. Dysregulation of Annexin A2 has been implicated in numerous diseases, such as cancer, inflammatory disorders, and autoimmune conditions, highlighting its importance as a potential biomarker for investigating these diseases and developing targeted therapies.
Overall, the Mouse ANXA2 CLIA Kit provides researchers with a valuable tool for studying the role of Annexin A2 in health and disease, offering reliable and informative data to advance scientific understanding and therapeutic interventions.
Product Name:
Mouse ANXA2 (Annexin A2) CLIA Kit
SKU:
MOES00077
Target:
Mouse ANXA2 (Annexin A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANXA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANXA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANXA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANXA2. You can calculate the concentration of Mouse ANXA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
102-115
101-117
Average (%)
91
109
107
1:4
Range (%)
98-114
98-112
97-111
Average (%)
107
105
104
1:8
Range (%)
92-106
90-104
85-100
Average (%)
98
96
91
1:16
Range (%)
99-114
99-113
92-106
Average (%)
105
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
107
EDTA plasma (n=5)
100-115
106
Cell culture media (n=5)
101-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
2.65
7.15
0.91
2.42
7.84
Standard deviation
0.09
0.22
0.57
0.11
0.17
0.7
C V (%)
9.0
8.3
7.97
12.09
7.02
8.93
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANXA2 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Mouse ANXA2 in samples. No significant cross-reactivity or interference between Mouse ANXA2 and analogues was observed.
