The Mouse ANXA5 (Annexin A5) CLIA Kit is a highly sensitive and specific assay designed for the precise detection of annexin A5 levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it perfect for a variety of research applications.Annexin A5 is a key protein involved in apoptosis and the regulation of blood coagulation. It plays a crucial role in various biological processes, including cell membrane repair, inflammation, and cancer progression.
The Mouse ANXA5 CLIA Kit allows researchers to study the function of annexin A5 in these processes and develop potential therapeutic interventions.Get your Mouse ANXA5 CLIA Kit today and unlock new insights into the role of annexin A5 in mouse models of disease. Visit www.assaygenie.com/mouse-anxa5-annexin-a5-clia-kit-moes00076/ for more information and to place your order.
Product Name:
Mouse ANXA5 (Annexin A5) CLIA Kit
SKU:
MOES00076
Target:
Mouse ANXA5 (Annexin A5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANXA5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANXA5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANXA5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ANXA5. You can calculate the concentration of Mouse ANXA5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-95
92-108
99-116
Average (%)
90
99
106
1:4
Range (%)
101-113
87-98
101-114
Average (%)
107
93
108
1:8
Range (%)
88-102
103-115
95-110
Average (%)
93
109
103
1:16
Range (%)
95-112
90-103
85-96
Average (%)
103
96
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
96-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.9
310.66
684.49
84.23
313.63
741.16
Standard deviation
8.93
29.82
75.98
10.05
29.14
46.84
C V (%)
9.72
9.6
11.1
11.93
9.29
6.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ANXA5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ANXA5 in samples. No significant cross-reactivity or interference between Mouse ANXA5 and analogues was observed.
