The Mouse APC (Activated Protein C) ELISA Kit is a comprehensive and reliable tool for measuring levels of APC in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and reproducible results for various research applications.Activated Protein C is an important regulator of blood clotting and inflammation, playing a critical role in maintaining vascular integrity and preventing excessive blood clotting.
Dysregulation of APC is associated with various diseases, including sepsis, thrombosis, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.With its advanced technology and user-friendly protocols, the Mouse APC ELISA Kit from Assay Genie is a valuable addition to any research laboratory seeking to explore the intricate mechanisms of blood coagulation and inflammation in mouse models.
Product Name:
Mouse APC (Activated Protein C) ELISA Kit
SKU:
MOES01803
Target:
Mouse APC (Activated Protein C) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse APC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse APC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse APC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse APC. You can calculate the concentration of Mouse APC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
97-113
87-99
Average (%)
96
103
92
1:4
Range (%)
89-101
88-98
86-98
Average (%)
95
93
92
1:8
Range (%)
89-102
83-93
86-97
Average (%)
96
89
91
1:16
Range (%)
89-102
81-90
82-92
Average (%)
96
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.01
563.69
1988.5
274.76
561.11
1860.49
Standard deviation
16.95
32.02
97.44
16.68
32.21
74.98
C V (%)
6.52
5.68
4.9
6.07
5.74
4.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse APC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse APC in samples. No significant cross-reactivity or interference between Mouse APC and analogues was observed.
