The Mouse APO-E (Apolipoprotein E) ELISA Kit is specifically designed for the precise detection of APO-E levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it perfect for various research purposes.APO-E is a vital protein crucial for lipid metabolism and plays a significant role in cardiovascular diseases, Alzheimer's disease, and other neurological disorders.
Studying APO-E levels can provide valuable insights into these conditions and aid in the development of potential treatments. With the Mouse APO-E ELISA Kit, researchers can confidently measure APO-E levels in mouse samples, facilitating in-depth investigations into its role in health and disease.
Product Name:
Mouse APO-E (Apolipoprotein E) ELISA Kit
SKU:
MOES00724
Target:
Mouse APO-E (Apolipoprotein E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoE. You can calculate the concentration of Mouse ApoE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
84-97
93-106
Average (%)
97
91
99
1:4
Range (%)
95-111
84-98
96-110
Average (%)
103
90
102
1:8
Range (%)
101-116
86-99
96-109
Average (%)
107
91
104
1:16
Range (%)
94-110
85-98
96-112
Average (%)
102
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.3
15.9
50.0
5.4
16.6
53.6
Standard deviation
0.4
0.7
2.2
0.3
1.0
2.2
C V (%)
7.55
4.4
4.4
5.56
6.02
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoE in samples. No significant cross-reactivity or interference between Mouse ApoE and analogues was observed.
