The Mouse APOM (Apolipoprotein M) ELISA Kit is specifically designed for the precise measurement of apolipoprotein M levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for various research applications.Apolipoprotein M is a key protein involved in lipid metabolism and high-density lipoprotein (HDL) function, playing a crucial role in maintaining cholesterol homeostasis.
Dysregulation of apolipoprotein M has been linked to various metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse APOM ELISA Kit provides researchers with a reliable tool for exploring the role of apolipoprotein M in metabolism and cardiovascular health, leading to a better understanding of its implications in disease pathology and potential treatment strategies.
Product Name:
Mouse ApoM (Apolipoprotein M) ELISA Kit
SKU:
MOES01281
Target:
Mouse ApoM (Apolipoprotein M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ApoM. You can calculate the concentration of Mouse ApoM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-116
88-103
86-102
Average (%)
106
95
93
1:4
Range (%)
92-109
82-94
83-99
Average (%)
99
88
90
1:8
Range (%)
90-104
81-92
82-93
Average (%)
97
87
88
1:16
Range (%)
93-105
82-93
84-96
Average (%)
98
86
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-96
91
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.53
4.31
0.49
1.58
3.88
Standard deviation
0.03
0.06
0.2
0.03
0.07
0.2
C V (%)
6.12
3.92
4.64
6.12
4.43
5.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ApoM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ApoM in samples. No significant cross-reactivity or interference between Mouse ApoM and analogues was observed.
