The Mouse AQP-2 (Aquaporin-2) CLIA Kit is a highly reliable and accurate tool for the quantitative detection of aquaporin-2 levels in mouse samples. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.Aquaporin-2 is a key protein involved in water reabsorption in the kidney, playing a critical role in maintaining water balance and urine concentration. Dysregulation of aquaporin-2 has been linked to various renal disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Mouse AQP-2 CLIA Kit, researchers can easily measure aquaporin-2 levels in serum, plasma, and tissue samples, providing valuable insights into renal function and water homeostasis in mouse models. Don't miss out on this essential tool for your research needs.
Product Name:
Mouse AQP-2 (Aquaporin 2) CLIA Kit
SKU:
MOES00095
Target:
Mouse AQP-2 (Aquaporin 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AQP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AQP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AQP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AQP-2. You can calculate the concentration of Mouse AQP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-97
85-100
87-99
Average (%)
90
92
93
1:4
Range (%)
91-105
85-101
85-98
Average (%)
96
92
92
1:8
Range (%)
87-101
96-114
101-118
Average (%)
93
104
109
1:16
Range (%)
97-111
92-106
99-113
Average (%)
104
100
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
100-112
106
Cell culture media (n=5)
101-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.54
9.72
1.2
2.74
9.51
Standard deviation
0.09
0.2
0.74
0.12
0.27
0.78
C V (%)
8.18
7.87
7.61
10.0
9.85
8.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AQP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AQP-2 in samples. No significant cross-reactivity or interference between Mouse AQP-2 and analogues was observed.
