The Mouse AQP-3 (Aquaporin 3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of AQP-3 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.Aquaporin 3 is a key protein involved in water transport and barrier function in the skin, kidneys, and other tissues. Dysregulation of AQP-3 has been implicated in various inflammatory skin disorders, kidney diseases, and cancer, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and user-friendly protocol, the Mouse AQP-3 CLIA Kit from Assay Genie is a valuable asset for researchers looking to explore the role of Aquaporin 3 in health and disease.
Product Name:
Mouse AQP-3 (Aquaporin 3) CLIA Kit
SKU:
MOES00097
Target:
Mouse AQP-3 (Aquaporin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AQP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AQP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AQP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AQP-3. You can calculate the concentration of Mouse AQP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
102-115
101-112
Average (%)
95
108
106
1:4
Range (%)
100-118
99-117
93-106
Average (%)
108
107
99
1:8
Range (%)
98-111
97-112
84-99
Average (%)
104
104
91
1:16
Range (%)
96-111
98-112
99-116
Average (%)
101
106
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
98-114
106
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.61
589.6
1737.39
217.38
634.83
1632.05
Standard deviation
23.51
56.25
109.98
24.69
70.02
169.73
C V (%)
11.11
9.54
6.33
11.36
11.03
10.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AQP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AQP-3 in samples. No significant cross-reactivity or interference between Mouse AQP-3 and analogues was observed.
