The Mouse ARG (Arginase) CLIA Kit is specifically designed for the sensitive detection of arginase levels in mouse serum, plasma, and cell culture supernatants. With high precision and accuracy, this kit provides reliable and consistent results, making it an essential tool for various research applications.Arginase is an important enzyme that plays a key role in the urea cycle and metabolism of arginine. It is involved in regulating nitric oxide production and has been implicated in various diseases such as cardiovascular disorders, autoimmune diseases, and metabolic syndromes.
Monitoring arginase levels can provide valuable insights into these conditions and aid in the development of potential therapies.Overall, the Mouse ARG CLIA Kit offers researchers a valuable tool for studying the role of arginase in health and disease, with its high sensitivity and specificity ensuring accurate measurement of arginase levels in mouse samples.
Product Name:
Mouse Arg (Arginase) CLIA Kit
SKU:
MOES00099
Target:
Mouse Arg (Arginase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Arg. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Arg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Arg, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Arg. You can calculate the concentration of Mouse Arg in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
86-97
87-98
Average (%)
101
91
93
1:4
Range (%)
89-103
99-114
95-112
Average (%)
96
105
103
1:8
Range (%)
88-102
94-110
94-107
Average (%)
94
101
100
1:16
Range (%)
85-96
96-110
88-102
Average (%)
91
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
96-112
104
Cell culture media (n=5)
89-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
0.84
3.88
0.52
0.87
4.24
Standard deviation
0.05
0.08
0.42
0.06
0.08
0.36
C V (%)
9.8
9.52
10.82
11.54
9.2
8.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Arg concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Arg in samples. No significant cross-reactivity or interference between Mouse Arg and analogues was observed.
