The Mouse ARNT2 (Aryl Hydrocarbon Receptor Nuclear Translocator 2) CLIA Kit is specifically designed for the accurate quantification of ARNT2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.ARNT2 is a critical transcription factor that plays a key role in various cellular processes, including the regulation of gene expression in response to environmental signals. Its involvement in pathways related to metabolism, development, and response to toxins makes it an important target for studying diseases such as cancer, metabolic disorders, and neurodegenerative conditions.
By using the Mouse ARNT2 CLIA Kit, researchers can accurately measure ARNT2 levels in biological samples, providing valuable insights into its function and potential implications in disease pathology. This kit is a valuable tool for advancing research in the field of molecular biology and drug discovery.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ARNT2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ARNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ARNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ARNT2. You can calculate the concentration of Mouse ARNT2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
100-119
90-105
Average (%)
91
108
96
1:4
Range (%)
84-97
92-108
94-104
Average (%)
90
99
99
1:8
Range (%)
102-116
85-99
93-107
Average (%)
108
91
99
1:16
Range (%)
103-115
92-106
95-109
Average (%)
109
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
84-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.12
217.35
723.9
104.85
221.97
782.43
Standard deviation
10.97
22.34
82.74
10.83
16.76
57.82
C V (%)
11.3
10.28
11.43
10.33
7.55
7.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ARNT2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ARNT2 in samples. No significant cross-reactivity or interference between Mouse ARNT2 and analogues was observed.
