The Mouse ATM (Ataxia Telangiectasia Mutated) CLIA Kit is a highly reliable and sensitive assay designed for the quantitative detection of ATM levels in mouse samples. This kit is ideal for researchers studying DNA damage response, cell cycle regulation, and the development of cancer therapies.ATM is a key protein involved in sensing and repairing DNA damage, crucial for maintaining genomic stability and preventing tumorigenesis. Dysregulation of ATM function has been implicated in various diseases, including cancer and neurodegenerative disorders.
By accurately measuring ATM levels in mouse samples, researchers can gain valuable insights into the role of ATM in disease pathogenesis and potential therapeutic interventions.With its high specificity and accuracy, the Mouse ATM CLIA Kit from Assay Genie offers a reliable and reproducible tool for investigating the biological functions of ATM in a research setting. Order yours today to advance your studies in DNA damage response and cancer research.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ATM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ATM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ATM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ATM. You can calculate the concentration of Mouse ATM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
99-115
91-106
Average (%)
109
105
99
1:4
Range (%)
94-105
96-108
89-99
Average (%)
99
101
94
1:8
Range (%)
85-100
99-114
97-107
Average (%)
92
106
102
1:16
Range (%)
95-109
88-102
96-112
Average (%)
101
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.24
41.33
157.32
21.41
44.05
149.42
Standard deviation
2.27
4.62
14.5
2.65
4.36
14.82
C V (%)
11.22
11.18
9.22
12.38
9.9
9.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ATM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ATM in samples. No significant cross-reactivity or interference between Mouse ATM and analogues was observed.
