The Mouse Axin2 (Axis Inhibition Protein 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Axin2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Axin2 is a key protein in the Wnt signaling pathway, regulating cell proliferation, differentiation, and stem cell maintenance. Dysregulation of Axin2 has been implicated in various diseases, including cancer, developmental disorders, and inflammatory conditions.
Therefore, measuring Axin2 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and user-friendly protocol, the Mouse Axin2 CLIA Kit from Assay Genie is a valuable tool for researchers studying the role of Axin2 in health and disease. Order yours today and unlock new possibilities in your research endeavors.
Product Name:
Mouse AXIN2 (Axis Inhibition Protein 2) CLIA Kit
SKU:
MOES00107
Target:
Mouse AXIN2 (Axis Inhibition Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AXIN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AXIN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AXIN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AXIN2. You can calculate the concentration of Mouse AXIN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
92-105
102-118
Average (%)
107
97
109
1:4
Range (%)
91-105
87-102
101-118
Average (%)
97
94
108
1:8
Range (%)
87-101
100-114
92-106
Average (%)
92
106
97
1:16
Range (%)
87-100
102-116
99-110
Average (%)
95
110
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
106
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.32
79.14
200.91
21.32
79.03
193.37
Standard deviation
2.37
6.58
15.71
2.7
8.94
22.2
C V (%)
10.16
8.31
7.82
12.66
11.31
11.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AXIN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AXIN2 in samples. No significant cross-reactivity or interference between Mouse AXIN2 and analogues was observed.
