The Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit is specifically designed for the quantitative detection of Bcl2 levels in mouse serum, plasma, and cell lysate samples. This kit offers high sensitivity and precision, providing researchers with reliable and reproducible results for their studies.Bcl2 is a key protein involved in regulating apoptosis, promoting cell survival, and maintaining the balance between cell death and proliferation. Dysregulation of Bcl2 has been implicated in various diseases, including cancer, autoimmune disorders, and cardiovascular diseases, highlighting its importance as a potential therapeutic target.
By using the Mouse Bcl2 ELISA Kit, researchers can accurately measure Bcl2 levels in mouse samples, allowing for a better understanding of its role in disease pathogenesis and the development of targeted treatments. This kit is an essential tool for studying Bcl2-related pathways and furthering research in the field of cell biology and immunology.
Product Name:
Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit
Product Code:
MODL00147
Size:
96 Assays
Target:
Bcl2
Synonyms:
B-Cell CLL/Lymphoma 2
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
91-102
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
