The Mouse BAFF (CD257) B-Cell Activating Factor CLIA Kit is a reliable and accurate assay designed for the quantification of BAFF levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing consistent and reproducible results for a variety of research applications.BAFF, also known as B-cell activating factor, is a key protein involved in the activation and survival of B-cells, playing a crucial role in the immune response.
Dysregulation of BAFF levels has been implicated in autoimmune diseases, such as lupus and rheumatoid arthritis, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.With its high performance and ease of use, the Mouse BAFF (CD257) B-Cell Activating Factor CLIA Kit is an essential tool for researchers investigating the role of BAFF in immune system function and disease pathogenesis.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BAFF/CD257 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAFF/CD257, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BAFF/CD257. You can calculate the concentration of Mouse BAFF/CD257 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
88-105
88-98
Average (%)
102
96
93
1:4
Range (%)
85-97
99-114
87-100
Average (%)
92
105
93
1:8
Range (%)
88-102
98-114
91-105
Average (%)
94
106
96
1:16
Range (%)
90-103
102-119
94-107
Average (%)
97
110
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
93-103
98
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.03
102.23
393.13
46.17
96.36
380.24
Standard deviation
5.24
8.26
30.11
4.98
11.41
23.77
C V (%)
10.91
8.08
7.66
10.79
11.84
6.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BAFF/CD257 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BAFF/CD257 in samples. No significant cross-reactivity or interference between Mouse BAFF/CD257 and analogues was observed.
