The Mouse BAFFR (B-cell Activation Factor Receptor) CLIA Kit is designed for the precise measurement of BAFFR levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.BAFFR is a key receptor involved in B-cell activation and survival, playing a critical role in the immune response. Dysregulation of BAFFR signaling has been linked to autoimmune diseases, such as lupus and rheumatoid arthritis, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its user-friendly format and reliable performance, the Mouse BAFFR CLIA Kit is a valuable tool for researchers seeking to further understand the role of BAFFR in immune regulation and disease pathogenesis.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BAFFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAFFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BAFFR. You can calculate the concentration of Mouse BAFFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
89-106
94-105
Average (%)
107
97
99
1:4
Range (%)
85-99
96-111
95-107
Average (%)
92
103
101
1:8
Range (%)
92-103
102-115
100-117
Average (%)
97
108
107
1:16
Range (%)
88-103
92-104
102-120
Average (%)
96
99
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
96-109
104
Cell culture media (n=5)
96-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.95
149.0
462.12
53.85
143.26
465.28
Standard deviation
6.42
14.84
27.96
6.06
14.37
34.2
C V (%)
12.85
9.96
6.05
11.25
10.03
7.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BAFFR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BAFFR in samples. No significant cross-reactivity or interference between Mouse BAFFR and analogues was observed.
