The Mouse BCL-2 (B-cell leukemia/lymphoma 2) CLIA Kit is a highly sensitive assay designed for the accurate detection of BCL-2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and reproducibility, ensuring reliable results for a variety of research applications.BCL-2 is a key regulator of apoptosis, playing a critical role in maintaining cell survival and preventing programmed cell death.
Dysregulation of BCL-2 has been implicated in various diseases, including cancer and autoimmune disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse BCL-2 CLIA Kit provides researchers with a powerful tool to investigate the role of BCL-2 in disease pathogenesis and to explore new avenues for targeted treatment strategies.
Product Name:
Mouse Bcl-2 (B-Cell Leukemia/Lymphoma 2) CLIA Kit
SKU:
MOES00112
Target:
Mouse Bcl-2 (B-Cell Leukemia/Lymphoma 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Bcl-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Bcl-2. You can calculate the concentration of Mouse Bcl-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
88-104
93-107
Average (%)
107
95
100
1:4
Range (%)
97-112
100-116
101-117
Average (%)
103
107
109
1:8
Range (%)
88-100
90-103
95-108
Average (%)
93
97
103
1:16
Range (%)
96-110
88-102
92-106
Average (%)
102
93
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
97-112
105
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.07
55.5
177.77
23.38
58.44
191.59
Standard deviation
3.05
3.92
12.69
2.78
5.16
21.71
C V (%)
12.67
7.06
7.14
11.89
8.83
11.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Bcl-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Bcl-2 in samples. No significant cross-reactivity or interference between Mouse Bcl-2 and analogues was observed.
