The Mouse Beta-CTX (Beta Crosslaps) ELISA Kit is specifically designed for the precise measurement of Beta Crosslaps levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research purposes.Beta Crosslaps is a key marker for bone resorption, providing valuable insights into bone metabolism and turnover. Understanding Beta Crosslaps levels can aid in the study of osteoporosis, bone disorders, and other skeletal conditions, offering potential avenues for therapeutic interventions.
With its reliable performance and comprehensive applications, the Mouse Beta-CTX ELISA Kit from Assay Genie is an invaluable tool for researchers seeking to delve deeper into bone health and related pathologies in mouse models. Visit www.assaygenie.com for more information on this cutting-edge assay kit.
Product Name:
Mouse beta-CTx (Beta Crosslaps) ELISA Kit
SKU:
MOES00928
Target:
Mouse beta-CTx (Beta Crosslaps)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-CTx. During the reaction, β-CTx in the sample or standard competes with a fixed amount of β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
88-100
89-104
Average (%)
102
95
97
1:4
Range (%)
85-96
87-100
100-116
Average (%)
91
94
106
1:8
Range (%)
85-101
85-98
94-107
Average (%)
92
92
101
1:16
Range (%)
82-95
92-107
96-110
Average (%)
89
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
264.2
768.9
1936.8
252.1
764.9
1935.4
Standard deviation
16.1
34.6
93.0
14.6
32.9
71.6
C V (%)
6.09
4.5
4.8
5.79
4.3
3.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of β-CTx concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes β-CTx in samples. No significant cross-reactivity or interference between β-CTx and analogues was observed.
