The Mouse BFP (Brain Finger Protein) CLIA Kit is a cutting-edge assay designed for the precise quantification of BFP levels in mouse brain tissue samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of experimental studies.BFP is a key protein involved in neurological functions, playing a critical role in synaptic plasticity and neuronal development. Dysregulation of BFP expression has been linked to various neurological disorders, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.
With its advanced technology and reliable performance, the Mouse BFP CLIA Kit is an essential tool for researchers studying brain function, neurodegenerative diseases, and synaptic transmission. Trust in this kit to deliver precise and reliable data for your valuable research endeavors.
Product Name:
Mouse BFP (Brain Finger Protein) CLIA Kit
SKU:
MOES00607
Target:
Mouse BFP (Brain Finger Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BFP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BFP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BFP. You can calculate the concentration of Mouse BFP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
86-99
89-100
Average (%)
108
93
95
1:4
Range (%)
103-120
89-99
96-111
Average (%)
109
94
102
1:8
Range (%)
88-98
103-120
85-98
Average (%)
93
110
91
1:16
Range (%)
95-109
98-111
99-115
Average (%)
102
105
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
105
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.17
224.64
796.65
113.26
240.31
867.9
Standard deviation
11.68
18.94
61.34
12.04
27.32
68.74
C V (%)
11.11
8.43
7.7
10.63
11.37
7.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BFP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BFP in samples. No significant cross-reactivity or interference between Mouse BFP and analogues was observed.
