Mouse BMP-1 (Bone Morphogenetic Protein 1) CLIA Kit
The Mouse BMP-1 (Bone Morphogenetic Protein 1) CLIA Kit is a comprehensive tool for the quantitative detection of BMP-1 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.BMP-1 is a key protein involved in bone development and maintenance, as well as in the regulation of extracellular matrix formation.
It plays a crucial role in processes such as osteogenesis, tissue repair, and organ development, making it a valuable biomarker for studying skeletal disorders and potential therapeutic interventions.Overall, the Mouse BMP-1 CLIA Kit provides researchers with a powerful tool to investigate the role of BMP-1 in various biological processes and diseases, offering a deeper understanding of its functions and potential implications for clinical applications.
Product Name:
Mouse BMP-1 (Bone Morphogenetic Protein 1) CLIA Kit
SKU:
MOES00121
Target:
Mouse BMP-1 (Bone Morphogenetic Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMP-1. You can calculate the concentration of Mouse BMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
93-105
96-110
Average (%)
92
98
102
1:4
Range (%)
85-100
88-102
95-109
Average (%)
92
95
102
1:8
Range (%)
87-100
87-100
96-109
Average (%)
93
92
103
1:16
Range (%)
90-103
84-99
90-105
Average (%)
96
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
101-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.69
124.12
414.0
51.01
120.12
394.8
Standard deviation
4.39
10.81
31.05
5.91
13.36
37.19
C V (%)
8.83
8.71
7.5
11.59
11.12
9.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP-1 in samples. No significant cross-reactivity or interference between Mouse BMP-1 and analogues was observed.
